Primers Available
Organism:Bactrocera cucurbitae mitochondrion
Repeat:(ATA)5
Start:7350 End:7364
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCAAAATTAGCTCCCAATCCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCAACTTTGGTGACTGCTGG | |||||||||||||
| Tm_F_1 | 59.533 | Size_F_1 | 21 | Tm_R_1 | 59.873 | Size_R_1 | 20 | PRODUCT Size | 134 bp | Primer Start | 7306 | Primer End | 7439 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAAATTAGCTCCCAATCCAGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGGAAATTATTGGGGCTTTG | |||||||||||||
| Tm_F_2 | 59.585 | Size_F_2 | 21 | Tm_R_2 | 59.767 | Size_R_2 | 20 | PRODUCT Size | 254 bp | Primer Start | 7308 | Primer End | 7561 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCAAAATTAGCTCCCAATCCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGGAAATTATTGGGGCTTTG | |||||||||||||
| Tm_F_3 | 59.533 | Size_F_3 | 21 | Tm_R_3 | 59.767 | Size_R_3 | 20 | PRODUCT Size | 256 bp | Primer Start | 7306 | Primer End | 7561 |