Primers Available
Organism: Ephemera orientalis mitochondrion
Repeat:(TTAT)3(TA)17*
Start:6064 End:6106
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGCATTCAGTTTCGACCTGAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGCAGCTCTCTTTTAATTACGGT | |||||||||||||
| Tm_F_1 | 58.857 | Size_F_1 | 21 | Tm_R_1 | 58.167 | Size_R_1 | 23 | PRODUCT Size | 173 bp | Primer Start | 6024 | Primer End | 6196 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCAAGGAGCCCTTAACTGAGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AACAGTGATAAGCCTCTTTTTGG | |||||||||||||
| Tm_F_2 | 59.721 | Size_F_2 | 21 | Tm_R_2 | 57.288 | Size_R_2 | 23 | PRODUCT Size | 228 bp | Primer Start | 5911 | Primer End | 6138 |
| FORWARD_PRIMER_3 | |||||||||||||
| CAAGGAGCCCTTAACTGAGCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AACAGTGATAAGCCTCTTTTTGG | |||||||||||||
| Tm_F_3 | 59.72 | Size_F_3 | 21 | Tm_R_3 | 57.288 | Size_R_3 | 23 | PRODUCT Size | 227 bp | Primer Start | 5912 | Primer End | 6138 |