Primers Available
Organism:Pyura gangelion complete mitochondrial genome
Repeat:(GTTT)3
Start:3456 End:3467
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTGGAGGAATTCGACAAAAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGGCATTTGCACCATAAAAA | |||||||||||||
| Tm_F_1 | 57.125 | Size_F_1 | 20 | Tm_R_1 | 59.936 | Size_R_1 | 20 | PRODUCT Size | 216 bp | Primer Start | 3432 | Primer End | 3647 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTGGAGGAATTCGACAAAAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CACTATGGCATTTGCACCAT | |||||||||||||
| Tm_F_2 | 57.125 | Size_F_2 | 20 | Tm_R_2 | 59.425 | Size_R_2 | 20 | PRODUCT Size | 221 bp | Primer Start | 3432 | Primer End | 3652 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTGGAGGAATTCGACAAAAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTCACTATGGCATTTGCACC | |||||||||||||
| Tm_F_3 | 57.125 | Size_F_3 | 20 | Tm_R_3 | 59.152 | Size_R_3 | 20 | PRODUCT Size | 223 bp | Primer Start | 3432 | Primer End | 3654 |