Primers Available
Organism:Rhizoctonia solani strain AG3 Rhs1AP mitochondrion
Repeat:(TATC)3
Start:185215 End:185226
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCGGTCAAACATTCGGTGTA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TATGGCTTCCAGTCACACCA | |||||||||||||
| Tm_F_1 | 59.964 | Size_F_1 | 20 | Tm_R_1 | 60.112 | Size_R_1 | 20 | PRODUCT Size | 203 bp | Primer Start | 185096 | Primer End | 185298 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACATCGGTCAAACATTCGGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TATGGCTTCCAGTCACACCA | |||||||||||||
| Tm_F_2 | 60.24 | Size_F_2 | 20 | Tm_R_2 | 60.112 | Size_R_2 | 20 | PRODUCT Size | 206 bp | Primer Start | 185093 | Primer End | 185298 |
| FORWARD_PRIMER_3 | |||||||||||||
| AACATCGGTCAAACATTCGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TATGGCTTCCAGTCACACCA | |||||||||||||
| Tm_F_3 | 60.755 | Size_F_3 | 20 | Tm_R_3 | 60.112 | Size_R_3 | 20 | PRODUCT Size | 207 bp | Primer Start | 185092 | Primer End | 185298 |