Primers Available
Organism:Rhizoctonia solani strain AG3 Rhs1AP mitochondrion
Repeat:(A)10
Start:176458 End:176467
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GATTTGCGGGAAACTTCTGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GAAAATAGGGACCACACGGA | |||||||||||||
| Tm_F_1 | 60.192 | Size_F_1 | 20 | Tm_R_1 | 59.79 | Size_R_1 | 20 | PRODUCT Size | 140 bp | Primer Start | 176407 | Primer End | 176546 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGCGGGAAACTTCTGAAAAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAAAATAGGGACCACACGGA | |||||||||||||
| Tm_F_2 | 59.685 | Size_F_2 | 20 | Tm_R_2 | 59.79 | Size_R_2 | 20 | PRODUCT Size | 136 bp | Primer Start | 176411 | Primer End | 176546 |
| FORWARD_PRIMER_3 | |||||||||||||
| GATTTGCGGGAAACTTCTGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATAATGCGCCTAAACCGTGT | |||||||||||||
| Tm_F_3 | 60.192 | Size_F_3 | 20 | Tm_R_3 | 59.501 | Size_R_3 | 20 | PRODUCT Size | 230 bp | Primer Start | 176407 | Primer End | 176636 |