Primers Available
Organism:Rhizoctonia solani strain AG3 Rhs1AP mitochondrion
Repeat:(T)12
Start:174291 End:174302
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GTGGATGCCGCTGTTATTTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAGTCCTCCACTTCTTGCCC | |||||||||||||
| Tm_F_1 | 59.967 | Size_F_1 | 20 | Tm_R_1 | 60.628 | Size_R_1 | 20 | PRODUCT Size | 204 bp | Primer Start | 174159 | Primer End | 174362 |
| FORWARD_PRIMER_2 | |||||||||||||
| GTGGATGCCGCTGTTATTTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGTGCTACAGCTCCCAAGTT | |||||||||||||
| Tm_F_2 | 59.967 | Size_F_2 | 20 | Tm_R_2 | 59.357 | Size_R_2 | 20 | PRODUCT Size | 246 bp | Primer Start | 174159 | Primer End | 174404 |
| FORWARD_PRIMER_3 | |||||||||||||
| GTGGATGCCGCTGTTATTTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCCCGTACGCGATATTTTT | |||||||||||||
| Tm_F_3 | 59.967 | Size_F_3 | 20 | Tm_R_3 | 59.942 | Size_R_3 | 19 | PRODUCT Size | 188 bp | Primer Start | 174159 | Primer End | 174346 |