Primers Available
Organism:Rhizoctonia solani strain AG3 Rhs1AP mitochondrion
Repeat:(T)11
Start:168005 End:168015
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCCAGGAATTGGGGTAATTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAGCAGGCATCGTAAGCAAT | |||||||||||||
| Tm_F_1 | 60.02 | Size_F_1 | 20 | Tm_R_1 | 59.873 | Size_R_1 | 20 | PRODUCT Size | 272 bp | Primer Start | 167860 | Primer End | 168131 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCCAGGAATTGGGGTAATTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGCAGGCATCGTAAGCAATC | |||||||||||||
| Tm_F_2 | 60.02 | Size_F_2 | 20 | Tm_R_2 | 60.382 | Size_R_2 | 20 | PRODUCT Size | 271 bp | Primer Start | 167860 | Primer End | 168130 |
| FORWARD_PRIMER_3 | |||||||||||||
| AATTAAGGCATCCGCACTTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGTTCAGCATCCTGCACTTA | |||||||||||||
| Tm_F_3 | 59.186 | Size_F_3 | 20 | Tm_R_3 | 60.011 | Size_R_3 | 20 | PRODUCT Size | 229 bp | Primer Start | 167958 | Primer End | 168186 |