Primers Available
Organism:Chrysomya rufifacies mitochondrion
Repeat:(ATTT)4
Start:13523 End:13538
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGAACATTATTTTTGCCGAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTTTATGGGATAAGCTGTAAAATAAAT | |||||||||||||
| Tm_F_1 | 58.304 | Size_F_1 | 20 | Tm_R_1 | 57.255 | Size_R_1 | 27 | PRODUCT Size | 260 bp | Primer Start | 13311 | Primer End | 13570 |
| FORWARD_PRIMER_2 | |||||||||||||
| CATGTTTTTGTTAAACAGGCGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTTTATGGGATAAGCTGTAAAATAAAT | |||||||||||||
| Tm_F_2 | 60.034 | Size_F_2 | 22 | Tm_R_2 | 57.255 | Size_R_2 | 27 | PRODUCT Size | 279 bp | Primer Start | 13292 | Primer End | 13570 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGTTTTTGTTAAACAGGCGAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTTATGGGATAAGCTGTAAAATAAAT | |||||||||||||
| Tm_F_3 | 58.391 | Size_F_3 | 21 | Tm_R_3 | 57.255 | Size_R_3 | 27 | PRODUCT Size | 277 bp | Primer Start | 13294 | Primer End | 13570 |