Primers Available
Organism: Ephemera orientalis mitochondrion
Repeat:(ATTA)3
Start:11590 End:11601
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCACGACCTGTAGAAGACCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCTCTTTATTGTGCTTTCAAAACACA | |||||||||||||
| Tm_F_1 | 60.109 | Size_F_1 | 20 | Tm_R_1 | 59.244 | Size_R_1 | 26 | PRODUCT Size | 164 bp | Primer Start | 11473 | Primer End | 11636 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCACGACCTGTAGAAGACCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACCTCTTTATTGTGCTTTCAAAACAC | |||||||||||||
| Tm_F_2 | 60.109 | Size_F_2 | 20 | Tm_R_2 | 59.243 | Size_R_2 | 26 | PRODUCT Size | 165 bp | Primer Start | 11473 | Primer End | 11637 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCACGACCTGTAGAAGACCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACCTCTTTATTGTGCTTTCAAAACA | |||||||||||||
| Tm_F_3 | 60.109 | Size_F_3 | 20 | Tm_R_3 | 58.127 | Size_R_3 | 25 | PRODUCT Size | 165 bp | Primer Start | 11473 | Primer End | 11637 |