Primers Available
Organism:Hemibarbus umbrifer mitochondrion
Repeat:(TTA)4
Start:10770 End:10781
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TAGCATTTGGTGCCACAGAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCCACCAGACTTTGTGACCT | |||||||||||||
| Tm_F_1 | 59.864 | Size_F_1 | 20 | Tm_R_1 | 60.002 | Size_R_1 | 20 | PRODUCT Size | 267 bp | Primer Start | 10703 | Primer End | 10969 |
| FORWARD_PRIMER_2 | |||||||||||||
| TAGCATTTGGTGCCACAGAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAAGCGGTTGGGAATATTGA | |||||||||||||
| Tm_F_2 | 59.864 | Size_F_2 | 20 | Tm_R_2 | 59.901 | Size_R_2 | 20 | PRODUCT Size | 234 bp | Primer Start | 10703 | Primer End | 10936 |
| FORWARD_PRIMER_3 | |||||||||||||
| GAAGCCACACTTATCCCGAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCCACCAGACTTTGTGACCT | |||||||||||||
| Tm_F_3 | 59.556 | Size_F_3 | 20 | Tm_R_3 | 60.002 | Size_R_3 | 20 | PRODUCT Size | 223 bp | Primer Start | 10747 | Primer End | 10969 |