Primers Available
Organism:Hemibarbus umbrifer mitochondrion
Repeat:(TCC)4
Start:14750 End:14761
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAATATTCACGCCAACGGAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGCCTATGTATGGGACTGCT | |||||||||||||
| Tm_F_1 | 59.96 | Size_F_1 | 20 | Tm_R_1 | 60.118 | Size_R_1 | 20 | PRODUCT Size | 233 bp | Primer Start | 14636 | Primer End | 14868 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAATATTCACGCCAACGGAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGGGTGTCGCCTATGTATGG | |||||||||||||
| Tm_F_2 | 59.96 | Size_F_2 | 20 | Tm_R_2 | 59.836 | Size_R_2 | 20 | PRODUCT Size | 240 bp | Primer Start | 14636 | Primer End | 14875 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACGTTGCACGAGGCCTATAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACAAATGGGAGCAGGAAGTG | |||||||||||||
| Tm_F_3 | 60.162 | Size_F_3 | 20 | Tm_R_3 | 60.111 | Size_R_3 | 20 | PRODUCT Size | 274 bp | Primer Start | 14686 | Primer End | 14959 |