Primers Available
Organism:Hemibarbus umbrifer mitochondrion
Repeat:(TA)6
Start:16488 End:16499
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCTTGTCAAACCCCAAAAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCATTGAGAAATTGCCCTAAA | |||||||||||||
| Tm_F_1 | 59.807 | Size_F_1 | 20 | Tm_R_1 | 59.202 | Size_R_1 | 21 | PRODUCT Size | 180 bp | Primer Start | 16408 | Primer End | 16587 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAACCCCAAAACCAAGGAAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCATTGAGAAATTGCCCTAAA | |||||||||||||
| Tm_F_2 | 60.193 | Size_F_2 | 20 | Tm_R_2 | 59.202 | Size_R_2 | 21 | PRODUCT Size | 172 bp | Primer Start | 16416 | Primer End | 16587 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGCAGCCTAGCAAGTTGAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCATTGAGAAATTGCCCTAAA | |||||||||||||
| Tm_F_3 | 59.752 | Size_F_3 | 20 | Tm_R_3 | 59.202 | Size_R_3 | 21 | PRODUCT Size | 141 bp | Primer Start | 16447 | Primer End | 16587 |