Primers Available
Organism:Botryllus schlosseri complete mitochondrial genome
Repeat:(T)12
Start:5880 End:5891
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTGGAAGGGTGGAATTTGAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACCCCCTCCCAATTAACATA | |||||||||||||
| Tm_F_1 | 59.903 | Size_F_1 | 20 | Tm_R_1 | 58.11 | Size_R_1 | 20 | PRODUCT Size | 279 bp | Primer Start | 5719 | Primer End | 5997 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTGGAAGGGTGGAATTTGAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TACCCCCTCCCAATTAACAT | |||||||||||||
| Tm_F_2 | 59.903 | Size_F_2 | 20 | Tm_R_2 | 58.11 | Size_R_2 | 20 | PRODUCT Size | 280 bp | Primer Start | 5719 | Primer End | 5998 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATCCGAGAAATTCTTGCCTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTCTAAAATACCCCCTCCCAA | |||||||||||||
| Tm_F_3 | 59.269 | Size_F_3 | 20 | Tm_R_3 | 59.661 | Size_R_3 | 21 | PRODUCT Size | 196 bp | Primer Start | 5811 | Primer End | 6006 |