Primers Available
Organism:Bursaphelenchus mucronatus mitochondrion
Repeat:(T)10(A)12
Start:6826 End:6847
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AATTTGAGAGTCCTCATTTATAGCTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCCTTTTAGGCAATTGATGG | |||||||||||||
| Tm_F_1 | 57.43 | Size_F_1 | 26 | Tm_R_1 | 59.406 | Size_R_1 | 20 | PRODUCT Size | 276 bp | Primer Start | 6733 | Primer End | 7008 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGGAGGTTGAATTTTAATTTGAGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTGATGGATAATTTGTACCGAAA | |||||||||||||
| Tm_F_2 | 59.067 | Size_F_2 | 24 | Tm_R_2 | 58.427 | Size_R_2 | 23 | PRODUCT Size | 278 bp | Primer Start | 6718 | Primer End | 6995 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGGAGGTTGAATTTTAATTTGAGAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTGATGGATAATTTGTACCGAAA | |||||||||||||
| Tm_F_3 | 59.9 | Size_F_3 | 25 | Tm_R_3 | 58.427 | Size_R_3 | 23 | PRODUCT Size | 278 bp | Primer Start | 6718 | Primer End | 6995 |