Primers Available
Organism:Hynobius nebulosus voucher CIB-XM3140 mitochondrion
Repeat:(T)11
Start:15980 End:15990
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GACATCCGCCTGAAGTATCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCGCACCGTTAGTTCAGATT | |||||||||||||
| Tm_F_1 | 59.513 | Size_F_1 | 20 | Tm_R_1 | 60.132 | Size_R_1 | 20 | PRODUCT Size | 154 bp | Primer Start | 15905 | Primer End | 16058 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGACATCCGCCTGAAGTATC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCGCACCGTTAGTTCAGATT | |||||||||||||
| Tm_F_2 | 60.624 | Size_F_2 | 20 | Tm_R_2 | 60.132 | Size_R_2 | 20 | PRODUCT Size | 155 bp | Primer Start | 15904 | Primer End | 16058 |
| FORWARD_PRIMER_3 | |||||||||||||
| GACATCCGCCTGAAGTATCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACCGCACCGTTAGTTCAGAT | |||||||||||||
| Tm_F_3 | 59.513 | Size_F_3 | 20 | Tm_R_3 | 59.621 | Size_R_3 | 20 | PRODUCT Size | 155 bp | Primer Start | 15905 | Primer End | 16059 |