Primers Available
Organism:Blastocystis sp. subtype 3 mitochondrion
Repeat:(AAT)5
Start:10267 End:10281
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTACCTTTATTAGCTGCTTTTCTTTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCAACTAATTTCATGTAAACCTTGA | |||||||||||||
| Tm_F_1 | 57.73 | Size_F_1 | 26 | Tm_R_1 | 58.605 | Size_R_1 | 25 | PRODUCT Size | 171 bp | Primer Start | 10230 | Primer End | 10400 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTACCTTTATTAGCTGCTTTTCTTTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACCAACTAATTTCATGTAAACCTTGA | |||||||||||||
| Tm_F_2 | 57.73 | Size_F_2 | 26 | Tm_R_2 | 59.323 | Size_R_2 | 26 | PRODUCT Size | 172 bp | Primer Start | 10230 | Primer End | 10401 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTACCTTTATTAGCTGCTTTTCTTTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACAAATTGGAAATAAAAAGGTTGT | |||||||||||||
| Tm_F_3 | 57.73 | Size_F_3 | 26 | Tm_R_3 | 57.3 | Size_R_3 | 24 | PRODUCT Size | 261 bp | Primer Start | 10230 | Primer End | 10490 |