Primers Available
Organism:Huperzia squarrosa mitochondrion
Repeat:(TTTC)3
Start:268954 End:268965
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAATGCCATAAAGCGCAAAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGGTCTGCCACATAACGTAG | |||||||||||||
| Tm_F_1 | 60.102 | Size_F_1 | 20 | Tm_R_1 | 59.22 | Size_R_1 | 20 | PRODUCT Size | 272 bp | Primer Start | 268752 | Primer End | 269023 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGCGTCATAGGTGTTTTTGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGGTCTGCCACATAACGTAG | |||||||||||||
| Tm_F_2 | 59.735 | Size_F_2 | 20 | Tm_R_2 | 59.22 | Size_R_2 | 20 | PRODUCT Size | 182 bp | Primer Start | 268842 | Primer End | 269023 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCAAACCAACATCCAGACAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGGTCTGCCACATAACGTAG | |||||||||||||
| Tm_F_3 | 59.547 | Size_F_3 | 20 | Tm_R_3 | 59.22 | Size_R_3 | 20 | PRODUCT Size | 258 bp | Primer Start | 268766 | Primer End | 269023 |