Primers Available
Organism:Huperzia squarrosa mitochondrion
Repeat:(AAAC)3
Start:232166 End:232177
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTCGAACCACTGACACAAGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCTTTCATTTCCGAATCCGT | |||||||||||||
| Tm_F_1 | 59.72 | Size_F_1 | 20 | Tm_R_1 | 59.505 | Size_R_1 | 20 | PRODUCT Size | 210 bp | Primer Start | 232101 | Primer End | 232310 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCGAACCACTGACACAAGGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCTTTCATTTCCGAATCCGT | |||||||||||||
| Tm_F_2 | 60.285 | Size_F_2 | 20 | Tm_R_2 | 59.505 | Size_R_2 | 20 | PRODUCT Size | 209 bp | Primer Start | 232102 | Primer End | 232310 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTTTTATTCTCCGCCTCCCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATTCGATGGTCCAGACATGC | |||||||||||||
| Tm_F_3 | 60.034 | Size_F_3 | 20 | Tm_R_3 | 60.904 | Size_R_3 | 20 | PRODUCT Size | 236 bp | Primer Start | 232023 | Primer End | 232258 |