Primers Available
Organism:Brassica oleracea mitochondrion
Repeat:(TTCT)3
Start:176715 End:176726
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTGGAAACTCGGGATCGTAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGGGTTCCTCCCTTGATTAT | |||||||||||||
| Tm_F_1 | 60.066 | Size_F_1 | 20 | Tm_R_1 | 60.145 | Size_R_1 | 20 | PRODUCT Size | 181 bp | Primer Start | 176611 | Primer End | 176791 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTGGAAACTCGGGATCGTAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAGTCACTGAAACGACGGGT | |||||||||||||
| Tm_F_2 | 60.066 | Size_F_2 | 20 | Tm_R_2 | 60.159 | Size_R_2 | 20 | PRODUCT Size | 196 bp | Primer Start | 176611 | Primer End | 176806 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCATTCTGATCGCCTCTTTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGGATAATCATTCCGGTCCT | |||||||||||||
| Tm_F_3 | 59.773 | Size_F_3 | 20 | Tm_R_3 | 59.981 | Size_R_3 | 20 | PRODUCT Size | 274 bp | Primer Start | 176644 | Primer End | 176917 |