Primers Available
Organism: Hynobius guabangshanensis mitochondrion
Repeat:(AAT)4
Start:4663 End:4674
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGGGGATGAGGCGGATTAAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGGGAGTCCGCCTAAGGATA | |||||||||||||
| Tm_F_1 | 59.523 | Size_F_1 | 20 | Tm_R_1 | 60.179 | Size_R_1 | 20 | PRODUCT Size | 273 bp | Primer Start | 4429 | Primer End | 4701 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGGATGAGGCGGATTAAACCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGGGAGTCCGCCTAAGGATA | |||||||||||||
| Tm_F_2 | 60.134 | Size_F_2 | 21 | Tm_R_2 | 60.179 | Size_R_2 | 20 | PRODUCT Size | 271 bp | Primer Start | 4431 | Primer End | 4701 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGGATGAGGCGGATTAAACC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGGGAGTCCGCCTAAGGATA | |||||||||||||
| Tm_F_3 | 58.394 | Size_F_3 | 20 | Tm_R_3 | 60.179 | Size_R_3 | 20 | PRODUCT Size | 271 bp | Primer Start | 4431 | Primer End | 4701 |