Primers Available
Organism:Brettanomyces custersianus mitochondrion
Repeat:(TTAT)3atagtattctattgatgaggatgttaattaattgtattaaatattcttaatttattaaaattactttt(TTTA)3
Start:4130 End:4221
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCAATTTACAGCTACTCATCATGTTA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGTGGAAGACAGATTGTCTAAACC | |||||||||||||
| Tm_F_1 | 57.256 | Size_F_1 | 26 | Tm_R_1 | 59.565 | Size_R_1 | 24 | PRODUCT Size | 275 bp | Primer Start | 4056 | Primer End | 4330 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACAGCTACTCATCATGTTAATTTAGAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCATACCTTCTGTGTGGAAGA | |||||||||||||
| Tm_F_2 | 57.019 | Size_F_2 | 27 | Tm_R_2 | 57.657 | Size_R_2 | 21 | PRODUCT Size | 280 bp | Primer Start | 4063 | Primer End | 4342 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCAATTTACAGCTACTCATCATGTTA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAACCATTTGACTTAAATTCGC | |||||||||||||
| Tm_F_3 | 57.256 | Size_F_3 | 26 | Tm_R_3 | 57.412 | Size_R_3 | 22 | PRODUCT Size | 256 bp | Primer Start | 4056 | Primer End | 4311 |