Primers Available
Organism:Blastocystis sp. DMP/02-328 mitochondrion
Repeat:(TAA)4
Start:22074 End:22085
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCCCAAATATGAAATGGTGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CATTTATTCTTCTGAAGCAGGTTT | |||||||||||||
| Tm_F_1 | 60.161 | Size_F_1 | 20 | Tm_R_1 | 58.119 | Size_R_1 | 24 | PRODUCT Size | 231 bp | Primer Start | 21912 | Primer End | 22142 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCCCAAATATGAAATGGTGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AACATTTATTCTTCTGAAGCAGGTTT | |||||||||||||
| Tm_F_2 | 60.161 | Size_F_2 | 20 | Tm_R_2 | 59.973 | Size_R_2 | 26 | PRODUCT Size | 233 bp | Primer Start | 21912 | Primer End | 22144 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCCCAAATATGAAATGGTGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACATTTATTCTTCTGAAGCAGGTTT | |||||||||||||
| Tm_F_3 | 60.161 | Size_F_3 | 20 | Tm_R_3 | 58.865 | Size_R_3 | 25 | PRODUCT Size | 232 bp | Primer Start | 21912 | Primer End | 22143 |