Primers Available
Organism:Blastocystis sp. DMP/02-328 mitochondrion
Repeat:(A)10
Start:27230 End:27239
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTCAGCACGTCGAAAAGTTG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATGCGTTCCACCTTTGAACT | |||||||||||||
| Tm_F_1 | 60.027 | Size_F_1 | 20 | Tm_R_1 | 59.598 | Size_R_1 | 20 | PRODUCT Size | 259 bp | Primer Start | 27009 | Primer End | 27267 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTCAGCACGTCGAAAAGTTG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TAACAGTCATGCGTTCCACC | |||||||||||||
| Tm_F_2 | 60.027 | Size_F_2 | 20 | Tm_R_2 | 59.572 | Size_R_2 | 20 | PRODUCT Size | 267 bp | Primer Start | 27009 | Primer End | 27275 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTCAGCACGTCGAAAAGTTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AACAGTCATGCGTTCCACCT | |||||||||||||
| Tm_F_3 | 60.027 | Size_F_3 | 20 | Tm_R_3 | 60.577 | Size_R_3 | 20 | PRODUCT Size | 266 bp | Primer Start | 27009 | Primer End | 27274 |