Primers Available
Organism: Batrachoseps wrightorum mitochondrion
Repeat:(G)20aacagacaaagcaaattcaaccca(CACC)3
Start:15567 End:15622
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACCCAAGGCCGAAATTCAAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGCCCAATTATAAGATGTAGGACA | |||||||||||||
| Tm_F_1 | 58.289 | Size_F_1 | 20 | Tm_R_1 | 57.236 | Size_R_1 | 24 | PRODUCT Size | 280 bp | Primer Start | 15479 | Primer End | 15758 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTTCCACTTCCACCCTCGAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGATGTTTATTATTGCTGTTGTCAACT | |||||||||||||
| Tm_F_2 | 60.037 | Size_F_2 | 20 | Tm_R_2 | 58.424 | Size_R_2 | 27 | PRODUCT Size | 195 bp | Primer Start | 15459 | Primer End | 15653 |
| FORWARD_PRIMER_3 | |||||||||||||
| GACTTCCACTTCCACCCTCG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGATGTTTATTATTGCTGTTGTCAACT | |||||||||||||
| Tm_F_3 | 60.037 | Size_F_3 | 20 | Tm_R_3 | 58.424 | Size_R_3 | 27 | PRODUCT Size | 197 bp | Primer Start | 15457 | Primer End | 15653 |